I want to have sextuplets, similar to Jon and Kate Gosseln:
But for now I am sticking to Neurons:
These are from one of my Gingko biloba cultures. The above neuron have strong growth but the bottom appears to be dying, which is indicated by the black bulges coming out of the neurites. This discrepcancy of growth is not irregular after 48 hours.
This one I shall inject into my first sextuplet
These next few pictures show how our measuring took place
Step 1: Open Original Picture under Adobe Photoshop
Step 2: Crop and edit Image so neurites stick out form background
Step 3: Using more Adobe photoshop techniques, further edit the image
Step 4: Threshold image and delete larger dark areas, such as nucleus, oil stains and erroneous black spots
Step 5: Open Image up in Image J. Have Image J analyze particles and then measure black area, which is the neuron area.
Funkafied image of neuron made in Adobe photoshop... Not to be used in analysis!!