Pseudocoloring Fluorescent Images - Axiovision Software

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Pseudocoloring Images - Axiovision Software

The cameras attached to the Zeiss microscopes are black and white cameras.  When you are using fluorescence, you will need to add color to your image (pseudocolor) after taking a picture:

1.    Left-click on the small color wheel at the bottom left of your open image so that it reads "on" instead of "off."
2.    In the small box to the right of the color wheel (with a number), right-click to bring up a list of colors.
3.    Left-click to select the color that is most similar to your fluorescence color.