Spring 2010 - BI474 Advanced Neurobiology

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Studied Four Species of Crustaceans

        - Cancer irroratus

        - Cancer borealis


Microdissection (removal of neural tissue)

        - Eyestalks

        - Pericardial Organs


         - Melatonin produced in crustacean eyestalks

        - Similar physiological and behavioral roles

        - Invertebrate receptors not yet studied

        - May have conserved role in cellular functions such as neurite growth

X-organ Cells:

        - Ease of culture

        - Similarity to vertebrate neurons

        - Survive and grow up to 7days

        - Multipolar

        - Neurosecretory (like vertebrate hypothalamus)

Sinus Gland

        - NDH

        - CHH

        - CAH

        - MIH

        - PDH

        - RPCH

Purpose and Goals

        - To identify areas of the crustacean nervous system with antidieuretic hormone, melatonin, and melatonin receptors

        - To learn techniques such as microdissection, cell culture, brightfield microscopy, fluorescence microscopy, confocal                     microscopy, and image analysis.

        - To apply these techniques to a collaborative group project.

        - To gain expertise in an area of neuroscience research; to design and conduct a research project; to analyze, interpret,               and present results.

        - To construct and update this website as an informative and instructive tool for the course.

        - To gain scientific skills in microdisection, immuncytochemistry, immunohistochemistry, flourescence and brightfield                     microscopy, confocal microscopy

Potential Clinical Implications

        - Neurodegenerative Diseases: Alzheimer’s, Parkinson’s, ALS

        - Axonal Regeneration

        - Nervous System Repair

        - Nerve damage, Paralysis