Spring 2010 - BI474 Advanced Neurobiology

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Introduction

Overview

-       Studied Four Species of Crustaceans

- Cancer irroratus

                        - Cancer borealis

                        - Uca Pugilator

                        - Homarus americanus

-       Microdissection (removal of neural tissue)

- Eyestalks

                        - Pericardial Organs

                        - Somatogastric Nervous System

                        - Brain

                        - Cardiac Ganglion

- MEL in Crustaceans (and other invertebrates)

            - Melatonin produced in crustacean eyestalks

- Similar physiological and behavioral roles

- Invertebrate receptors not yet studied

- May have conserved role in cellular functions such as neurite growth

- X-organ Cells:

- Ease of culture

- Similarity to vertebrate neurons

- Survive and grow up to 7days

- Multipolar

- Neurosecretory (like vertebrate hypothalamus)

            - Sinus Gland

                   - NDH

- CHH

- CAH

- MIH

- PDH

- RPCH

Purpose and Goals

-       To identify areas of the crustacean nervous system with antidieuretic hormone, melatonin, and melatonin receptors

-       To learn techniques such as microdissection, cell culture, brightfield microscopy, fluorescence microscopy, confocal microscopy, and image analysis.

-       To apply these techniques to a collaborative group project.

-       To gain expertise in an area of neuroscience research; to design and conduct a research project; to analyze, interpret, and present results.

-       To construct and update this website as an informative and instructive tool for the course.

-       To gain scientific skills in microdisection, immuncytochemistry, immunohistochemistry, flourescence and brightfield microscopy, confocal microscopy

-       Potential Clinical Implications

o     Neurodegenerative Diseases: Alzheimer’s, Parkinson’s, ALS

o     Axonal Regeneration

o     Nervous System Repair

o      Nerve damage, Paralysis

Methods

Dissections

GENERAL DISSECTION

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