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Comment: Migrated to Confluence 4.0

This is me!


                                                                                                                                                             I want to have sextuplets, similar to Jon and Kate Gosseln: 
 


                                          

But for now I am sticking to Neurons:

       

These are from one of my Gingko biloba cultures.  The above neuron have strong growth but the bottom appears to be dying, which is indicated by the black bulges coming out of the neurites.  This discrepcancy of growth is not irregular after 48 hours.                                                                                                               
                                                                 
                                                                  

This one I shall inject into my first sextuplet 
                                                                                                                                           


These next few pictures show how our measuring took place
 
 

 
 
 
 
 
 
 
         
 
 
             Step 1: Open Original Picture under Adobe Photoshop
 
 
 
 
 
 
 
 
 

 

 
 
 
 
 
 
 
 
 
 
 
          Step 2: Crop and edit Image so neurites stick out form background
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
           Step 3: Using more Adobe photoshop techniques, further edit the image
 
 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 
  Step 4: Threshold image and delete larger dark areas, such as nucleus, oil stains and erroneous black spots

 
 
 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 Step 5: Open Image up in Image J. Have Image J analyze particles and then measure black area, which is the neuron area.
 
 
 
 
 
 
 
 
 
 
 


 
Funkafied image of neuron made in Adobe photoshop... Not to be used in analysis!!